Unraveling the secrets of poloxamer 188 - A webinar mystery


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When an inexplicable drop in cell culture performance is linked to a batch of media, researchers race to find the culprit.

Join us to unravel the mystery as we journey through the structure, synthesis, and diabolical byproducts of poloxamer 188. We'll unmask the culprit -- and the RP-LC/MS method we developed to prevent it from striking again!  

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On Demand - Unraveling the secrets of poloxamer 188 - A webinar mystery  


Details

Presenter

Damien A. Bellos, PhD
Damien A. Bellos, PhD
Senior Analytical Scientist
Cytiva


Abstract

Polypropylene glycol (PPG) with a 1000-2000 Da molecular weight range was recently identified as a cytostatic impurity in poloxamer 188 (P188), demonstrating a need for enhanced PPG screening measures. PPG and high molecular weight poloxamer species. (HMW) are known byproducts of P188 synthesis and can decrease the growth of some CHO cultures at very low concentrations. 

Here we'll discuss our work to identify the impact of these impurities, and the reversed-phase liquid chromatography and mass spectrometry (LC/MS) method we developed to screen for them in a single test. We'll present data demonstrating the feasibility of this approach and our results since implementing it.

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