For more information, see Montero-Llopis et al., 2021.

1. Which image modality are you using?

Widefield

Spinning Disk Confocal

Point Scanning Confocal

Multiphoton

Did you use excitation/emission filter wheels or filter cubes?

Filter Wheel

Filter Cube

What light source(s) did you use to illuminate your sample?

Non-laser light source (e.g., LED, metal halide)

Lasers

2. Did you acquire transmitted light images (e.g., phase contrast, brightfield, DIC)?

Yes

No

3. Did you use additional magnification (e.g., optovar, relay lens)?

Yes

No

4. Did you perform any of these multidimensional acquisitions?

Multi-color

Z-stack

Time-lapse

5. Did you do a multi-point acquisition (e.g., revisiting different positions in a time-lapse)?

Yes

No

6. Did you do tiling, scan large area, or mosaic acquisition?

Yes

No

7. Did you do live cell imaging?

Yes

No, my sample is fixed

8. What kind of fluoropohore(s) did you use in your sample?

Fluorescent protein

Fluorescent dye/organic dye

9. Did you use any transfection reagents to express the fusion protein of interest in your sample?

Yes

No

10. Did you use use microscopes in a core facility?

Yes

No

App developed by Rebecca Senft (2021).

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